The ViPrimePLUS Peste-des-petits-ruminants Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Peste-des-petits-ruminants Virus (PPRV) genome in discharge from eyes, nose and mouth, stool and blood samples.
Peste-des-petits-ruminant disease is a highly contagious disease that often affects goats and sheep but no symptoms in cattle. PPRV is a single stranded RNA virus that is transmitted via aerosol, direct contact and contaminated feed. The virus is shed in secretion and excretion of the infected animals. There are 3 manifestation of the disease, i.e. acute, peracute and subacute forms. The sudden onset of fever, depression and watery discharge are common signs of infection followed by diarrhea, breathing difficulty and pneumonia. In later stage, formation of small nodular lesions in skin and around the muzzle can be observed. Mortality rate in susceptible animal populations can vary from 50-100%, making it as one of the most economically significant veterinary pathogens for small ruminants.
ViPrimePLUS Peste-des-petits-ruminants Virus RT-qPCR Kit is intended for research use only.
- PPRV Primers and Probe Mix (PPRV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
||Taqman probe-based real-time PCR assay
|Type of Nucleic Acid Kit
||Shipped in room temperature, store at -20℃ on arrival
||Ruminant samples (e.g. discharge from eyes, nose and mouth, stool, blood samples)
||Large protein gene
||10 to <100 copies per reaction
|Sensitivity & Specificity
||Ct value 28±3 with probability of 95%
||• Internal Extraction Control (IEC) as inhibition control
• Positive control
||• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
||ViPrimePLUS Peste-des-petits-ruminants Virus RT-qPCR Kit
ViPrimePLUS Peste-des-petits-ruminants Virus RT-qPCR Kit