Amino acid sequence
MKHHHHHHAS FSWDNCDEGK DPAVIRSLTL EPDPIIVPGN VTLSVMGSTS VPLSSPLKVD LVLEKEVAGL WIKIPCTDYI GSCTFEHFCD VLDMLIPTGE PCPEPLRTYG LPCHCPFKEG TYSLPKSEFV VPDLELPSWL TTGNYRIESV LSSSGKRLGC IKIAASLKGI.
GM2AP1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ser33-Ile193) containing 170 amino acids including a 9 aa His tag at N-terminus. The total calculated molecular mass is 18.7kDa.
GM2AP1 was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M Tris buffer and 0.1M NaCl, pH 8.0.
Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. GM2AP1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
GM2 Ganglioside Activator, Cerebroside Sulfate Activator Protein, Sphingolipid Activator Protein 3, GM2-AP, SAP-3, GM2 Ganglioside Activator Protein, Shingolipid Activator Protein 3, GM2A.