Bio-Helix

Blultra prestained protein standard pm001 0500

彩色標記蛋白質電泳標準品 / BLUltra Prestained Protein Ladder(6.5 to 270 kDa)

型號: PM001-0500
規格: 500 μl
產地: 台灣
報價: 2,450 元
運費: 以PDF報價單為準
交期: 期貨,依供應商說明
供應商: 良知股份有限公司
聯絡電話: 02-26518190

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詳細 資訊

The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer).  The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
 

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of BLUltra Prestained Protein Ladder in different electrophoresis conditions are listed below :

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2~3 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.


Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
* supplement data should be considered for more accurate adjustment. All products are for research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses.

 

 

Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That's why we're pleased to launch the color-enhanced BLUltra prestained protein standards.

As you may see the comparison data shown below, BLUltra shows a better resolution than the BLUeye & BlueRAY for the protein bands from 95 kDa to 270 kDa.

Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PM001-0500 BLUltra Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PM007-0500 BLUeye Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PM001-0500 BLUltra Prestained Protein Ladder (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PM007-0500 BLUeye Prestained Protein Ladder (Bio-Helix) / 10μl