Vivantis

Vivantis

PCR聚合酶 / Taq DNA Polymerase (5u/ul)

型號: PL1202
規格: 500 units
產地: 馬來西亞
報價: 1,406 元
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交期: 期貨,下個月到貨
供應商: 良知股份有限公司
聯絡電話: 02-26518190

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詳細 資訊

Taq DNA Polymerase (Thermus aquaticus)

Description
Taq DNA Polymerase is a thermostable DNA polymerase. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+ and has the 5' to 3'exonuclease activity.

Features

  • Ultra pure recombinant protein allows amplification up to 8kb.
  • 10X ViBuffer S provided for amplification of more than 5kb amplicon.
  • Generates mostly 3' dA overhang amplification products which are suitable for TA cloning.

Unit Definition
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ ml BSA in a final volume of 50μl.

Supplied With

  • 10X ViBuffer A (without MgCl2)
    500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP
  • 10X ViBuffer S 
    160mM (NH4)2SO4, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
  • 50mM MgCl2

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.

Ordering Information

Catalog No Description Pack Size
PL1202 Taq DNA Polymerase 500u, 5u/μl
PL1204 Taq DNA Polymerase 2 X 1000u, 5u/μl

Download
Manual

Taq DNA Polymerase

Comparison Test Report

Taq DNA Polymerase

Publication
This Product Has Been Used In: 

AL,S.,Hizlisoy, H., Ertas, N., Yildrim,Y., GÖNÜLALAN, Z. (2016) Occurrence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, and Typhi isolated from chicken eggs and poultry products. Turkish Journal of Veterinary and Animal Sciences. 40. pp.737-743 

Chumgwangwapee, S., Chingsungnoen, A., Siri, S. (2016) A plasma modified cellulose-chitosan porous membrane allows efficient DNA binding and provides antibacterial properties: A step towards developing a new DNA collecting card. Forensic Science International: Genetics.25. pp.19-25 

Jarukamjorn, K. et al. (2016) A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice Scientificap. 1-10. 

Laopichienpong, N., Muangmai, N., Supikamolseni, A.,Twilprawat, P., Chanhome L., Suntrarachun, S., Peyachoknagul, S., Srikulnath, K. (2016). Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand. . Gene. 594(2).pp.238-247.

Laohakiet, K., et al. (2016) The Study of the transformer Gene from Bactrcera dorsalis and B. correcta with Putative Core Promoter Regions BMC Genetics17(34), p.1-17. 

Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. LWT - Food Science and Technology.73. Pp592-601 

Martinez-Guzman, M.A., Alvarado-Navarro,A., Pereira-Suarez,A.L., Muñoz-Valle, J.F., Fafutis-Morris,M. (2016) Association between STR -794 CATT5-8 and SNP -173 G/C polymorphisms in the MIF gene and Lepromatous Leprosy in Mestizo patients of western Mexico. Human Immunology. 29 (3). 77(10) pp.985-989

Rosli, M.K.A., et al (2016) Phylogenetic Relationships of the Vulnerable Wild Cattle, Malayan gaur (Bos gaurus hubbacki), and its Hybrid, the Selembu, Based on Maternal Markers Turkish Journal of Zoologyp.1-10. 

Ruzilawati, A.B., et al (2016) α-4 Subunit of Nicotinic Acetylcholine Receptor Polymorphisms Exhibit No Association with Smoking Behavior among Malay Males in Kelantan, Malaysia The Egyptian Journal of Medical Human GeneticsScienceDirect. 17, p.71-77. 

Singh, R., et al. (2016) )From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network. Plant Science. 242. Pp.278-287. 

Allahverdi, A., et al. (2015) Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1 CELL Journal. S17(2), p.231-242. 

Celiani, F., Soler, L., Grilli, G., Marques, A.T., Giudice, C., Lecchi, C. (2015) The localization and differential expression of Serum Amyloid A in bovine liver and adipose tissue depots. Veterinary Immunology and Immunopathology. 168.pp35-39. 

Devran, Z., et al. (2015) Development of Molecular Markers Tightly Linked to Pvr4 Gene in Pepper Using Next-Generation Sequencing Molecular Breeding. 35(101), p.1-9. 

Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels Virology Journal12(102), p. 1-10. 

Muangsuwan, W., Ruangsuj, P., Chaichanachaicharn, P., Yasawong, M. (2015). A novel nucleic lateral flow assay for screening of PHA-producing haloarchaea. Journal of Microbiological Methods.116. Pp.8-14

Moghassem Hamidi, R., et al. (2015) Association between the Enterotoxin Production and Presence of Coa, Nuc Genes among Staphylococcus aureus Isolated from Various Sources, in Shiraz. Iranian Journal of Veterinary Research. 16(4), p.381-384. 

Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490. 

Onmaz, N.E., Abay, S., Karadal, F., Hizlisoy,. Telli, N., Al S. (2015). Occurence and antimicrobial resistance of Staphylococcus aureus and Salmonella spp. in retail fish samples in Turkey. Marine Pollution Bulletin. 90,(1-2). pp242-246 

Rahman, M.M., Lecchi, C., Sauerwein, H., Mielenz, M., Häußler, S., Restelli, L., Giudice, C., Ceciliani,F. (2015) Expression of α1-acid glycoprotein and lipopolysaccharide binding protein in visceral and subcutaneous adipose tissue of dairy cattle. The Veterinary Journal. The Veterinary Journal. 203. Pp223-227. 

Restelli, L., Lecchi, C., Invernizzi, G., Avallone, G., Savoini, G., Ceciliani,F. (2015) UCP1 and UCP2 expression in different subcutaneous and visceral adipose tissue deposits in 30 days old goat kids and effect of fatty acid enriched diets. . Research in Veterinary Science.100.pp.131-137 

Sezer, C. et al. (2015)Food Handlers: A Bridge in the Journey of Enterotoxigenic MRSA in FoodJournal of Consumer Protection and Food Safety10, p. 123-129. 

Thakur,S., et al. (2015) Extensive Sequence Variation in Rice Blast Resistance Gene Pi54 Makes It Broad Spectrum in NatureFrontiers in Plant Science. 6(345), p.1-14. 

Abushama, H.M., et al. (2014)Antibody Response to Cutaneous Leishmaniasis in Sudan.The International Journal of Biotechnology, 3(10), p.123-137. 

Aketarawong, N., Isasawin, S., & Thanaphum, S. (2014) Evidence of Weak Genetic Structure and Recent Gene Flow between Bactrocera dorsalis s.s. and B. papaya, across Southern Thailand and West Malaysia, supporting a single target pest for SIT applications. BMC Genetics15(70). 

Hassan, M.M., Zain, H.M., Basheer, M.A., Elhaj, H.E.F., El-Sayed, B.B. (2014) Swarming and mating behavior of male Anopheles arabiensis Patton(Diptera: Culicidae) in an area of the Sterile Insect Technique Projectin Dongola, northern Sudan. . Acta Tropica. 132s. pp.S64-S69. 

Masstor, N.H., et al. (2014) Molecular Phylogeny of the Bamboo Sharks (Chiloscyllium spp.). BioMed Research International. 

Sahilah, A.M. et al (2014) Antiobiotic Resistance and Molecular Typing among Cockle (Anadara granosa) Strains of Vibrio parahaemolyticus by Polymerase Chain Reaction (PCR)-based Analysis World Journal of Microbiology and Biotechnology. ProQuest30, p. 649-659. 

Sharif, S., et al. (2014)Multiplex PCR in Determination of Opiinae parasitoids of Fruit Flies, Bactrocera sp., Infesting Star Fruit and Guava Journal of Insect Science14(7), p. 1-14. 

Cha, T.S., et al. (2013)Identification and Characterization of RAPD-SCAR Markers Linked to Glyphosate-Susceptible and –Resistant Biotypes of Eleusine indica (L.) GaertnMolecular Biology Reports. 

Nazree, N.E., et al. (2013)Lack of Association Between TPH2 Gene Polymorphisms with Major Depressive Disorder in Multiethnic Malaysia Population. Asia-Pacific Psychiatry p.1-6. 

Waiho, K. et al (2013) Isolation and Characterization of Partial Mitochondrial CO1 Gene from Harpacticoid Copepod, Leptocaris canariensis (Lang, 1965). Academic Journal, 12(50): 6901-6906 

Dilda, F., Pisani, L.F., Rahman, M.Md, Modina, S., Tessaro, I., Sartorelli, P., Ceciliani, F., lecchi,C. (2012) Distribution of acute phase proteins in the bovine forestomachs and abomasums . The Veterinary Journal.192. pp.101-105

Charoenporn, C., et al (2010) Evaluation of Bio-agent Formulations to Control Fusarium Wilt of Tomato. African Journal of Biotechnology ProQuest. 9(36), p. 5836-5844. 

Ertas, N., Gonulalan, Z., Yildirim, Y., Kum, E. (2010) Detection of Staphylococcus aureus enterotoxins in sheep cheese and dairy desserts by multiplex PCR technique. International Journal of Food Microbiology.142. Pp.74-77. 

Devran and Söğüt. (2009) Distribution and Identification of Root-knot Nematodes from Turkey. J Nematol. 41(2): 128–133.