Bio-Helix

Mb101 0500 taq dna polymerase

Taq DNA聚合反應酵素 / Taq DNA Polymerase

型號: MB101-0500
規格: 500 units
產地: 台灣
報價: 2,225 元
運費: 以PDF報價單為準
交期: 期貨,依供應商說明
供應商: 良知股份有限公司
聯絡電話: 02-26518190

  • 報價金額的單位為新台幣。
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詳細 資訊

Taq DNA Polymerase is purified from E. coli. expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and a 5′ → 3′ exonuclease activity but lacks a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. Taq Polymerase is recommended for use in routine PCR reactions. The buffer system is optimized for high specificity and guarantees minimal by-product formation. We supplied Taq Polymerase with appropriate buffers. Usually 1-1.5 u of Taq DNA Polymerase are used in 50 µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA Polymerase (2-3 u) may be necessary to obtain a better yield of amplification products.