Amino acid sequence
MKHHHHHHASQEDGFEGTEE GSPREFIYLN RYKRAGESQD KCTYTFIVPQ QRVTGAICVN SKEPEVLLEN RVHKQELELL NNELLKQKRQ IETLQQLVEV DGGIVSEVKL LRKESRNMNS RVTQLYMQLL HEIIRKRDNA LELSQLENRI LNQTADMLQL ASKYKDLEHK YQHLATLAHN QSEIIAQLEE HCQRVPSARP VPQPPPAAPP RVYQPPTYNR IINQISTNEI QSDQNLKVLP PPLPTMPTLT SLPSSTDKPS GPWRDCLQAL EDGHDTSSIY LVKPENTNRL MQVWCDQRHD PGGWTVIQRR LDGSVNFFRN WETYKQGFGN IDGEYWLGLE NIYWLTNQGN YKLLVTMEDW SGRKVFAEYA SFRLEPESEY YKLRLGRYHG NAGDSFTWHN GKQFTTLDRD HDVYTGNCAH YQKGGWWYNA CAHSNLNGVW YRGGHYRSRY QDGVYWAEFR GGSYSLKKVV MMIRPNPNTF H.
ANGPTL2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 481 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 56kDa (calculated).
ANGPTL2 was filtered (0.4 µm) and lyophilized from 0.5mg/ml in 0.03M Acetate buffer pH 4.0.
Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, an intensive dilution by relevant buffer to a concentration of 10?g/ml is recommended. In higher concentrations the solubility of the ANGPTL2 is limited. ANGPTL2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Angiopoietin-related protein 2, Angiopoietin-like protein 2, ANGPTL2, ARP2, HARP.