Vivantis

Vivantis

qPCR反應試劑套組 / ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit

型號: QV7003
規格: 1 set
產地: 馬來西亞
報價: 57,813 元
運費: 以PDF報價單為準
交期: 期貨,下個月到貨
供應商: 良知股份有限公司
聯絡電話: 02-26518190

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詳細 資訊

ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit

Description
The ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Israeli Acute Paralysis Virus genome in bee samples.

Israeli Acute Paralysis Virus (IAPV) is a single stranded RNA virus which kills bees and causes colony collapse disorder. The virus is widespread in the Middle East, Australia, USA and also in Europe. IAPV is transmitted by the mite Varroa destructor that feeds on the bodily fluids of adult, pupal and larval bees. Symptoms of infection include shivering wings, progressing to paralysis, and then death outside of the hive. The phylogenetic analysis reveals 3 distinct lineages of IAPV circulating the world, i.e.; 1) group 1 which represents western USA and Australia samples, 2) group 2 which represents Israel samples and 3) group 3 which represents eastern USA and Canada samples. Appearance of IAPV-resistant bees is the product of incorporation of the viral genes into the bee genome mechanism.

ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit is intended for research use only.

Kit Component

  • IAPV Primers and Probe Mix (IAPV PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Bee samples
Gene Target Helicase gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QV7003 ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit 150 reactions

Download
Manual

ViPrimePLUS Israeli Acute Paralysis Virus RT-qPCR Kit