ProSpec

Prospec 1

重組蛋白質 / Gliadin Gamma 18.6kDa Wheat; Gliadin Gamma 18.6kD Wheat Recombinant

型號: PRO-114
規格: 1mg
產地: 以色列
報價: 155,901 元
運費: 以PDF報價單為準
交期: 期貨,下個月到貨
供應商: 灃德生物科技有限公司
聯絡電話: 0988-461219

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詳細 資訊

Applications

Western blot with monoclonal anti-hexa-His-tag antibody.

Coating concentration

0.15-0.5 µg/ml (depending on the type of ELISA plate and coating buffer).

Description

Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.

Formulation

Gliadin is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl, and 20% glycerol.

Immunological function

Binds IgG- and IgA-type human autoantibodies associated with celiac disease.

Immunological tests

Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot.

Physical Appearance

Sterile Filtered clear solution.

Purity

Greater than 94% as determined by SDS-PAGE.

Source

Escherichia Coli.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Link

http://www.prospecbio.com/Gliadin_Wheat_10