ProSpec

Prospec 1

細胞因子 / IFN a 1b Human; Interferon-Alpha 1b Human Recombinant

型號: CYT-283
規格: 1mg
產地: 以色列
報價: 35,978 元
運費: 以PDF報價單為準
交期: 期貨,下個月到貨
供應商: 灃德生物科技有限公司
聯絡電話: 0988-461219

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詳細 資訊

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.

Biological Activity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.

Description

Interferon-alpha 1b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton.The Interferon-alpha 1b gene was obtained from human leukocytes.The IFN-a 1b is purified by proprietary chromatographic techniques.

Formulation

Lyophilized from a 0.2µm filtered solution (50ug/ml) in PBS, pH 7.4, containing 4% mannitol and 1% HSA.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized IFN alpha 1b in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Interferon alpha 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 1b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Link

http://www.prospecbio.com/Interferon-alpha-1b_Human_3